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Allylamine-mediated DNA attachment to polycrystalline diamond surface

( 2009-10-19) Zhuang, H. ; Srikanth, Vadali V.S.S. ; Jiang, X. ; Luo, J. ; Ihmels, H. ; Aronov, I. ; Wenclawiak, B. W. ; Adlung, M. ; Wickleder, C.

Allylamine, an unsaturated short carbon chain amine was used to mediate ss-DNA attachment to an H-terminated polycrystalline diamond thin film surface for biosensoric applications. At first, allylamine was photochemically tethered onto the diamond film surface; ss-DNA was then attached via the allylamine linkage. The DNA molecules are then hybridized with the complementary DNA molecules containing fluorescence labels followed by denaturing. Time-of-fight secondary ion mass spectrometry and fluorescence spectroscopy are used to confirm the allylamine bonding and the covalent DNA bonding to the diamond film surface, respectively. © 2009 American Institute of Physics.